Bibliography of Author: Makabe, K.W.

  1. Kawashima, T., Kawashima, S., Kanehisa, M., Nishida, H., and Makabe, K.W.. "MAGEST: MAboya gene expression patterns and sequence tags." Nucleic Acids Res. 28 (1). 2000. pp. 133-5.
    [ .pdf ] [ PubMed ]

    MAGEST is a database for newly identified maternal cDNAs of the ascidian, Halocynthia roretzi, which aims to examine the population of the mRNAs. We have collected 3' and 5' tag sequences of mRNAs and their expression data from whole-mount in situ hybridi-zation in early embryos. To date, we have determined more than 2000 tag-sequences of H.roretzi cDNAs and input them into public databases. The tag sequences and the expression data as well as additional information can be obtained through MAGEST via the WWW at http://www.genome.ad.jp/magest/

    Keywords: DNA Complementary ; *Databases Factual ; *Expressed Sequence Tags ; *Gene Expression ; Information Storage and Retrieval ; Urochordata_*genetics


  2. Kawashima, T., Kawashima, S., Kohara, Y., Kanehisa, M., and Makabe, K.W.. "Update of MAGEST: Maboya Gene Expression patterns and Sequence Tags." Nucleic Acids Res. 30 (1). 2002. pp. 119-20.
    [ .pdf ] [ PubMed ]

    MAGEST is a database for maternal gene expression information for an ascidian, Halocynthia roretzi. The ascidian has become an animal model in developmental biological research because it shows a simple developmental process, and belongs to one of the chordate groups. Various data are deposited into the MAGEST database, e.g. the 3'- and 5'-tag sequences from the fertilized egg cDNA library, the results of similarity searches against GenBank and the expression data from whole mount in situ hybridization. Over the last 2 years, the data retrieval systems have been improved in several aspects, and the tag sequence entries have increased to over 20 000 clones. Additionally, we constructed a database, translated MAGEST, for the amino acid fragment sequences predicted from the EST data sets. Using this information comprehensively, we should obtain new information on gene functions. The MAGEST database is accessible via the Internet at http://www.genome.ad.jp/magest/.

    Keywords: Amino Acid Sequence ; DNA Complementary_genetics ; *Databases Genetic ; *Expressed Sequence Tags ; Forecasting ; *Gene Expression Regulation ; Developmental ; Gene Library ; In Situ Hybridization ; Information Storage and Retrieval ; Internet ; RNA Messenger Stored_biosynthesis ; Urochordata_*embryology ; Urochordata_*genetics ; Urochordata_metabolism ; Zygote_metabolism